A Basis of the Stomatocytoses of Erythrocytes by 1-Chloro-2, 4-Dinitrobenzene and Other Electrophilic Reagents
Identifiers and Pagination:Year: 2015
First Page: 16
Last Page: 19
Publisher Id: BIOLSCI-1-16
Article History:Received Date: 29/1/2015
Revision Received Date: 15/3/2015
Acceptance Date: 5/5/2015
Electronic publication date: 26/6/2015
Collection year: 2015
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
On bases of previous observations on the band 3 anion exchange inhibition by the electrophilic reagent 1-fluoro-2,4-dinitrobenzene in resealed ghosts and a previously proposed band 3-based mechanism of control of the erythrocyte shape, it is suggested that stomatocytoses by 1-chloro-2,4-dinitrobenzene and other electrophilic reagents are due to an inhibition of the band 3 anion exchange by a covalent modification of Lys 539, one of the two lysine intramolecularly cross-linked by impermeant 4,4’-diisothiocyanodihydrostilbene-2,2’- disulfonic acid, a band 3 anion exchange potent inhibitor. Attempts to explain these stomatocytoses led to explain the echinocytosis and competitive inhibition of band 3 anion exchange by stilbenedisulfonic acid derivatives and the reversal of the echinocytosis by 2,4-dinitrophenol by one stomatocytogenic of the electrophilic reagents. The inference on stomatocytoses by electrophilic reagents is of interest since stomatocytogenic and echinocytogenic by amphiphiles are perceived to interact non-covalently with the membrane and that they can be clinically relevant.
The electrophilic reagent 1-chloro-2,4-dinitrobenzene (CDNB) at a relative low concentration (0.5-2 mM) in an isotonic buffered saline depletes the erythrocyte glutathione within a period of 60 min at 37 o C by a dinitrophenylation of its cysteine residue by the intermediary of glutathione S-transferase [1-3]. This depletion is accompanied by a K+ leakage, a stomatocytosis and a decrease of the erythrocyte deformability , and a negligible hemolysis  becoming prominent at a higher CDNB concentration (5 mM) [2, 4]. This stomatocytosis was noticed during a survey of a literature in order to test the validity of a previously proposed band 3-based mechanism of control of the erythrocyte shape . It appeared to be due to dinitrophenylations of membrane proteins and aminophospholipids since it was observed after cell washing . However, it appeared unrelated to dinitrophenylations of cysteine residues of membrane proteins since alkylations of these cysteine residues by N-ethylmaleimide (5-9.5 mM) or iodoacetamide (9.5 mM) opposed to echinocytosis by salicylate  and to stomatocytosis by chlorpromazine or Triton-X-100 [6, 7], apparently by reducing the skeleton flexibility. The band 3-based mechanism did not appear to be able to explain this stomatocytosis as well as other echinocytoses and stomatocytoses by other agents, including the echinocytosis by glucose depletion. However, recently, a process of echinocytosis by glucose depletion has been proposed based on this mechanism  and the following previous observations on ghosts and erythrocytes with other electrophilic reagents have drawn our attention, which led us to a second attempt to explain the stomatocytosis by CDNB by this mechanism. The structural analogue 1-fluoro-2,4-dinitrobenzene (FDNB) inhibited the band 3 anion exchange in resealed ghosts at a relatively low concentration (0.15-0.3 mM) [7-10]. The affinity labels of proteases N-(-tosyl-L-phenylalanine-chloromethyl ketone (TPCK) and N-(-tosyl-L-lysine-chloromethyl ketone (TLCK) and one of the impurities of the insecticide bromfenvinphos, 2,4-dichlorophenacyl bromide, induced stomatocytoses under similar conditions with the exception of a higher TLCK concentration required (4 mM) to induce it, presumably being due to be more polar [11-14]. These observations suggested that stomatocytoses by CDNB and other electrophilic reagents have a common basis and result from a covalent modification of band 3. Attempts to explain these stomatocytoses also led to explain observations related to stilbenedisulfonic acid derivatives, which are potent inhibitors of the band 3 anion exchange, a reversal of an echinocytosis by the amphiphile 2,4-dinitrophenol (DNP) amphiphile by the electrophilic reagent TPCK.
The band 3-based mechanism of a control of the erythrocyte shape has the major following features. Band 3 (AE1, SLC4A1) is a 90-100 kDa transmembrane glycoprotein which rapidly exchanges chloride (Cl-) and bicarbonate (HCO3-). It is bound to ankyrin R which binds in the mid region of the flexible filamentous tetrameric anionic spectrin. The latter forms a two-dimensional skeleton with band 4.1 R and actin. Band 4.1 R and actin form ternary complexes at the two ends of filamentous tetrameric anionic spectrin. Actin forms protofilaments binding 5-6 molecules of band 4.1 R which is bound to the transmembrane glycoprotein C. Band 3 also transports endogenous inorganic phosphate (Pi) (its dibasic form is transported with a hydrogen ion (H+)) and a wide variety of other inorganic and organic anions, including bulky ones, but at a slow rate . The obligatory alternative recruitment of its inward-facing (band 3i) and outward-facing (band 3o) conformations in exchanges of Cl- and HCO3- folds and unfolds spectrin (or contracts and relaxes the skeleton), thus promoting echinocytosis and stomatocytosis, respectively. The band 3o/ band 3i equilibrium ratio increases with the increase of the Donnan equilibrium ratio of anions Cl- and HCO3- and H+ (r = Cl-i /Cl-o = HCO3-i/HCO3-o = H+o/H+i), which is determined by hemoglobin and the major organic phosphate intermediate 2,3-bisphophoglycerate (2,3-BPG). Substrates slowly transported by band 3, preferentially inwardly and outwardly are echinocytogenic and stomatocytogenic, respectively.
An examination of observations on the inhibition of the band 3 anion exchange by FDNB (0.15-0.3 mM) in resealed ghosts at 37oC shows that this inhibition is due to a dinitro-phenylation of Lys 539, one of the two lysine residues intra-molecularly cross-linked by impermeant 4,4’-diisothiocya-nodihydrostilbene-2,2’- disulfonic acid (H2DIDS) (Lys 539, Lys 851), a high affinity inhibitor of the band 3 anion exchange [7, 9, 16]. Lys 539 is not essential of the band 3 anion exchange although is nearby of the transport site [16-18]. Impermeant H2DIDS initially binds non-covalently to band 3 when its outward-facing conformation (band 3o) is recruited or when the band 3o/band 3i ratio increases , which recruitment is stomatocytogenic according to the band 3-based mechanism. However, a recruitment of the band 3 inward-facing conformation subsequently occurs according to this mechanism, as indicated by an echinocytosis by impermeant covalently modifying 4,4’-diisothiocyanodihy-drostilbene-2,2’- disulfonic acid (DIDS) (0.01-0.02 mM, 37oC) [20, 21], or non-covalently modifying 4,4’-dinitrostilbene-2,2’- disulfonic acid (DNDS) (2.5 mM, 22oC) . The degree of echinocytosis by DNDS correlates with the degree of band 3 saturation without observing a sphero-echinocytosis after band 3 saturation, indicating that the echinocytosis is due to the inhibition of the band 3 anion exchange. The inference of a stomatocytosis followed by an echinocytosis by a stilbenedisulfonic acid derivative is supported by previous stopped-flow kinetics of binding of covalently modifying DIDS or non-covalently modifying 4,4’-dibenzaminostil-bene-2,2’- disulfonic acid (DBDS) to band 3 in ghosts which show that there is initially a fast phase, followed by a slower phase of its binding, which are dependent and independent of their concentrations, respectively [22, 23]. The inhibition of the band 3 transport of Cl- by a stilbenedisulfonic acid derivative is competitive although this derivative does not fully inhibit Cl- binding to the transport site, as indicated by Cl- binding to band 3 in ghosts by chloride-35 nuclear magnetic resonance (Cl35-NMR) spectroscopy [23, 24]. It is attributed either to the binding of the stilbenedisulfonic acid derivative to the transport site  or to an allosteric site . However, the following observations would support the view that the derivative binds to the transport site. Echinocytogenic stilbenedisulfonic acid derivatives DNDS and DBDS  are slowly transported by band 3 and substrates slowly inwardly transported by band 3 are echinocytogenic. Erythrocytes in a suspension at a low hematocrit (0.02 %) in a low ionic strength of an isotonic sucrose solution buffered by sodium phosphate, pH 5.2 undergo a rapid morphological transformation from echinocytes to stomatocytes . The presence of these echinocytocytes can be attributed to an inward transport of Pi by band 3, favored by acid pH of the medium and an increase of the band 3o/band 3i ratio by increasing the Donnan ratio, whereas the transformation of echinocytes to stomatocytes can be attributed to the outward transport of Pi transport by band 3. However, the covalent modifying stilbenedisulfonic acid derivative DIDS (0.004 mM) only delays this morphological transformation.
Impermeant H2DIDS reacts with Lys 539 at neutral pH without cross-linking with Lys 851, which occurs at alkaline pH . The preferential covalent modification of Lys 539 by H2DIDS or FDNB is attributed to its high reactivity owing to its low pKa of 8.5 which is approximately two units lower pKa of a normal lysine [7, 9]. The Cl35-NMR spectroscopy on the inhibition of the band 3 anion exchange of Cl- by FDNB in ghosts shows that this inhibition is due to an absence of the access of Cl- to the transport site , which could be attributed to an alteration of the three-dimensional structure of the transport site by the covalent modification of Lys 539 since this lysine is close to this site. The absence of the access of Cl- to the transport site by inhibition of the band 3 anion exchange by FDNB would be stomatocytogenic according to the band 3-based mechanism of control of the erythrocyte shape since the skeleton could not be contracted by decreasing the band 3o/band 3i ratio in the absence of Cl- binding to the transport site. Consequently, it can be inferred that the stomatocytoses by the electrophilic reagents CDNB, TPCK, TLCK or 2,4-dichlorophenacyl bromide are due to an inhibition of the band 3 anion exchange by a covalent modification of Lys 539 which would increase the band 3o/band 3i ratio. A stomatocytosis by the covalent modification of Lys 539 not essential of the band 3 anion exchange would raise the possibility that this lysine influences the erythrocyte shape by altering the band 3o/band 3i ratio.
The echinocytosis of erythrocytes by DNP (1 mM) is reversed at 37oC by a prior treatment of erythrocytes at 4oC for 1 h by TPCK at a sub-stomatocytogenic concentration (0.05 mM) (4 times lower) (3.7 % disc recovery/min versus 0.16 % disc recovery/min of the control) in a Ringer solution buffered with N-2-hydroxyethylpiperazine-N`-ethanesulfonic acid (Hepes) supplemented with MgCl2, Pi and glucose followed by a cell washing [11, 12]. Its reversal by TPCK would be explained as follows. A small fraction of band 3 molecular species in each discocyte are irreversibly modified by a stomatocytogenic covalent modification of Lys 539 by TPCK at a low concentration without a visible stomatocytosis. This small modification, which by itself would relax the skeleton by increasing the band 3o/band 3i ratio, would reverse the echinocytosis by allowing an inward translocation of a certain fraction of DNP molecular species in the inner leaflet which would increase the band 3o/band 3i ratio by increasing the inner leaflet surface area, thereby further relaxing the skeleton. Alternatively, DNP molecular species inwardly translocated increases the band 3o/band 3i ratio by their outward transport by band 3. This fraction may be relatively small as suggested by this observation on an erythrocyte shape transition. Sphero-echinocytes formed by monopalmitoylphosphatidylcholine (0.07 mM) in a phosphate buffer saline supplemented with glucose are transformed to discocytes over a period of 7.5 h at 37oC, which are transformed to stomatocytes by a selective extraction of monopalmitoylphosphatidylcholine molecular species in the outer leaflet with serum albumin . This reversal transformation involves an inward translocation of a fraction of 0.13 of amphiphile molecular species in the outer leaflet which would be attributed to the increase of the band 3o/band 3i ratio by the outward transport of Pi by band 3 promoted by glucose metabolism . The echinocytosis by dimyristoylphosphatidylcholine, accompagnied by a vesiculation, was inhibited by TLCK at a stomatocytogenic concentration (4 mM) . The explanation of this inhibition is likely to be basically the same as that of the reversal of the echinocytosis by DNP by TPCK at a sub-stomatocytogenic concentration.
The inference that stomatocytoses by CDNB and other electrophilic reagents are due to an inhibition of the anion exchange by the covalent modification of Lys 539 of band 3 would be interest since stomatocytogenic and echinocyto-genic amphiphiles are perceived to interact non-covalently with the membrane. Moreover, it can be clinically relevant since it raises the possibility that stomatocytosis in alcoholism, which can be induced in blood samples by acetaldehyde (1 mM, 2 h at 4oC) , the first metabolite produced in the hepatic ethanol metabolism, is due to a covalent modification of Lys 539 by this metabolite. Moreover, it was previously observed that an inherited deficiency of glucose-6-phosphate dehydrogenase, the enzyme catalyzing the first and rate limiting step of the pentose phosphate pathway, prevented the reversal of the echinocytosis by DNP by TPCK, indicating of an alteration of the membrane, which was also suggested by a particularly slow reversal of the echinocytosis by DNP by the activator of the pentose phosphate pathway amphiphilic methylene blue not altering the erythrocyte shape . It has been previously shown that the phosphorylation of the band 3 cytoplasmic domain is increased after cysteine oxidation and increasing salt concentration in glucose-6-phosphate dehydrogenase deficiency erythrocytes .
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